mcd99Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40In the case of mCD99, two adjacent O- glycosylation sites, Thr-45 and Thr-50 (NMKPT 45 PKAPT 50 PKKPS) are relevant for PILR ␣ recognition (13). Sequence alnment of human